1，宿主 Host: Escherichia coli (E. coli)
2，种类Species: Human
3，蛋白类型Protein Type: Recombinant
4，别名Synonyms: CG6988, Dmel\\CG6988, Dpdi, EP(3)1022, PDI, dpdi, pdi, XPDIp, DSI, GIT, PHDB, PDIA1, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta, Thbp, ERp59, Pdia1, PDIR, p55
5，纯度Purity: 95% by SDS-PAGE
6, 应用范围Application: WB， Elisa， Functional Studies
7.背景Background:
Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E. coli is a single, non-glycosylated, polypeptide chain containing 508 AA and having a molecular mass of 57.1 kDa. The human PDI contains N-terminal 6xHis-tag and is purified by proprietary chromatographic techniques.
8保存和稳定性Storage&Stability:
Avoid repeated freeze and thaw cycle.Store at 2-8℃ for delivery and store at -20 or -70℃ for future use. The loss rate of the target protein which describes the stability was determined by accelerated thermal degradation test.That is to incubate the protein at 37℃ for 48h and no obvious degradation and precipitation were observed.The loss of this protein is less than 5% within the expiration date under appropriate storage condition.